Optimal timing for the oral administration of Da-Cheng-Qi decoction based on the pharmacokinetic and pharmacodynamic targeting of the pancreas in rats with acute pancreatitis

Table 1 Concentrations of the 10 components of DCQD distributed in pancreatic tissues

	NG(a)	4hG(a)	12hG(a)	24hG(a)
Rhein	12.65 ± 3.7	179.15 ± 77.6a	199.89 ± 34.8	50.08 ± 17.6e
Emodin	18.68 ± 8.7	1.33 ± 0.8a	34.46 ± 10.3c	39.75 ± 12.4
Naringin	415.30 ± 17.8	19.47 ± 1.4a	32.10 ± 2.7c	269.16 ± 12.9e
Honokiol	2.38 ± 1.2	1.98 ± 0.1	19.79 ± 2.2c	4.26 ± 1.0
Magnolol	1.34 ± 0.9	0.97 ± 0.2	0.97 ± 0.2	7.51 ± 0.8e
Hesperidin	162.01 ± 34.3	34.94 ± 12.6a	24.31 ± 10.3	113.26 ± 13.8e
Naringenin	847.98 ± 76.9	858.58 ± 19.6	1077.06 ± 26.8c	262.30 ± 100.6e
Aloe-emodin	439.05 ± 179.8	89.53 ± 14.8a	502.74 ± 70.7c	501.45 ± 143.4
Chrysophanol	60.99 ± 16.4	5.16 ± 2.1a	197.29 ± 17.9c	113.43 ± 23.7e
Rheochrysidin	6.79 ± 1.2	0.55 ± 0.2a	17.92 ± 1.9c	11.67 ± 0.9e

Rats were orally administered with DCQD (10 mL/kg.BW). Pancreatic tissues were collected 24 h after a single dosage of DCQD. The concentrations of the 10 components of DCQD were determined by HPLC-MS/MS. The results are presented as the mean ± SD of μg/mg (n = 6). NG(a): the sham-operated group with the dosing time at 4 h after operation. 4hG(a), 12hG(a) and 24hG(a): rats were dosed orally with DCQD at 4 h, 12 h and 24 h, respectively, after AP induction. 4hG(a) vs NG(a):

^aP < 0.05, 12hG(a) vs 4hG(a):

^cP < 0.05, 24hG(a) vs 12hG(a):

^eP < 0.05. AP: Acute pancreatitis; DCQD: Da-Cheng-Qi decoction; HPLC-MS/MS: High-performance liquid chromatography tandem mass spectroscopy.