Detection of KRAS G12D in colorectal cancer stool by droplet digital PCR

Table 3 KRAS G12D mutation levels in DNA from stool samples of wild-type control patients

				G12D								Wild-type
				Single				Merged				Single					Merged
Patient	Tumor location	Tumor stage	Positive events		Copies/μL	Copies/20 μL reaction	Positive events		Copies/μL	Copies/20 μL reaction	Copies/ng DNA		Positive events	Copies/μL	Copies/20 μL reaction	Positiveevents		Copies/μL	Copies/20 μL reaction	Copies/ng DNA
53	Left	I	1		0.1	2	3		0.07	1.40	0.011		94	9	180	469		10.50	210.00	1.615
			2		0.17	3.4							12	10.8	216
			0		0	0							116	9.9	198
			0		0	0							136	12.1	242
56	Left	IIA	1		0.08	1.6	1		0.02	0.40	0.003		17	1.4	28	76		1.56	31.20	0.240
			0		0	0							25	2.1	42
			0		0	0							16	1.3	26
			0		0	0							18	1.4	28
63	Right	IIA	1		0.08	1.6	1		0.02	0.42	0.003		258	20.4	408	957		20.30	406.00	3.123
			0		0	0							222	22.1	442
			0		0	0							217	19	380
			0		0	0							260	19.9	398
71	Right	IIIB	1		0.08	1.6	3		0.06	1.20	0.009		89	6.7	134	377		7.20	144.00	1.108
			2		0.15	3							104	7.9	158
			0		0	0							93	6.9	138
			0		0	0							91	7.3	146
96	Left	IV	1		0.09	1.8	3		0.06	1.20	0.009		32	3	60	133		2.84	56.80	0.437
			1		0.08	1.6							33	2.6	52
			1		0.09	1.8							34	3	60
			0		0	0							34	2.9	58