Copyright
©The Author(s) 2017.
World J Gastroenterol. Oct 14, 2017; 23(38): 6995-7008
Published online Oct 14, 2017. doi: 10.3748/wjg.v23.i38.6995
Published online Oct 14, 2017. doi: 10.3748/wjg.v23.i38.6995
Figure 7 Mfn2 knockdown relieves mitochondrial Ca2+ overloading.
Rhod-2 AM was used to detect mitochondrial Ca2+ concentrations by confocal microscopy and VarioskanFlash. A: Confocal microscopy confirmed that the vast majority of Rhod-2 AM fluorescence was associated with the mitochondria; B: VarioskanFlash was used to detect Rhod-2 AM to determine the fluorescence of mitochondrial Ca2+ concentrations. Taking the hypoxia group as the reference, both the NC and hypoxia + Si groups relieved mitochondrial Ca2+ overloading. Mfn2: Mitofusin-2; NC: Normal culture; Rhod-2 AM: Rhod-2 acetoxymethyl ester. cP < 0.001.
- Citation: Liang RP, Jia JJ, Li JH, He N, Zhou YF, Jiang L, Bai T, Xie HY, Zhou L, Sun YL. Mitofusin-2 mediated mitochondrial Ca2+ uptake 1/2 induced liver injury in rat remote ischemic perconditioning liver transplantation and alpha mouse liver-12 hypoxia cell line models. World J Gastroenterol 2017; 23(38): 6995-7008
- URL: https://www.wjgnet.com/1007-9327/full/v23/i38/6995.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i38.6995