Mitofusin-2 mediated mitochondrial Ca2+ uptake 1/2 induced liver injury in rat remote ischemic perconditioning liver transplantation and alpha mouse liver-12 hypoxia cell line models

Figure 6 Mnf2 knockdown attenuates apoptosis in AML-12 hypoxia cell lines. The Annexin V/PI Apoptosis Detection Kit by flow cytometry (A and C); Caspase 3/7 detection kit and tetramethylrhodamine methyl ester perchlorate by confocal microscopy (B) used to detect apoptosis. The ratio of apoptosis cells was lower in both the NC and hypoxia + Si groups. AML: Alpha mouse liver; Mfn2: Mitofusin-2; NC: Normal culture. ^aP < 0.05.