Adipose-derived stromal cells resemble bone marrow stromal cells in hepatocyte differentiation potential in vitro and in vivo

Figure 2 RT-PCR, western blot analysis and immunofluorescence staining of hepatocyte-related markers following hepatic induction. A: Gene expression of albumin (ALB), alpha fetoprotein (AFP), cytokeratin 18 (CK-18), CK-19, and glucose-6-phosphate (G-6-P) was significantly enhanced in hepatocyte-like cells on day 10 following hepatic differentiation. Undifferentiated cells served as a negative control; B: Western blot analysis of hepatocyte-related markers in adipose-derived stromal cells (ADSCs), bone marrow stromal cells (BMSCs), and hepatocyte-like cells; C: Immunofluorescence staining of hepatocyte-related markers in ADSCs, BMSCs, and hepatocyte-like cells. On day 10 after differentiation, immunofluorescence staining revealed that the differentiated hepatocyte-like cells expressed the hepatocyte markers ALB, CK-19, CK-18, AFP and CYP1A1. Scale bar: 200 μm.