Mitofusin-2 mediated mitochondrial Ca2+ uptake 1/2 induced liver injury in rat remote ischemic perconditioning liver transplantation and alpha mouse liver-12 hypoxia cell line models

doi:10.3748/wjg.v23.i38.6995

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 14, 2017; 23(38): 6995-7008
Published online Oct 14, 2017. doi: 10.3748/wjg.v23.i38.6995

Table 1 Primer sequences for qRT-PCR

Target gene	Primer sequence
Rat-β-Actin	Forward: 5'-ACGGTCAGGTCATCACTATCG-3'
Rat-β-Actin	Reverse: 5'-GAGGTCTTTACGGATGTCAACG-3'
Rat-Mfn2	Forward: 5'-GAAGAAGAGTGTCAAGACCGTG-3'
Rat-Mfn2	Reverse: 5'-CAGGCAAAACTTATCAATCCAG-3'
Rat-Mcu	Forward: 5'-GACCCTGAACGATGTGAAGAC-3'
Rat-Mcu	Reverse: 5'-TTCTCCGCTTTCCTGCTAAT-3'
Rat-MICU-1	Forward: 5'-GACTAAGCGGAGACTGATGTTG-3'
Rat-MICU-1	Reverse: 5'-GAGATTCTGCGTGAGCCTTC-3'
Rat-MICU-2	Forward: 5'-ACAAAGCCTCACTCTGGGT-3'
Rat-MICU-2	Reverse: 5'-TCACTGTTGGTTCCTGGTATT-3'
Mus-β-Actin	Forward: 5'-CATTGCTGACAGGATGCAGAAGG-3'
Mus-β-Actin	Reverse: 5'-TGCTGGAAGGTGGACAGTGAGG-3'
Mus-Mfn2	Forward: 5'-CAAGTGTCCGCTCCTGAAG-3'
Mus-Mfn2	Reverse: 5'-CCACCAGCACAAACACATC-3'
Mus-Mcu	Forward: 5'-GAGCCGCATATTGCAGTACGGT-3'
Mus-Mcu	Reverse: 5'-AAACACGCCGACTGAGTCAGAG-3'
Mus-MICU-1	Forward: 5'-GAAGTGTCCAGCCGTGAAGGAA-3'
Mus-MICU-1	Reverse: 5'-TGGTGTGGAGTAGGCTCGGATT-3'
Mus-MICU-2	Forward: 5'-GGAAGACTTTGCTATCGCCATGC-3'
Mus-MICU-2	Reverse: 5'-GGTGTCCAGAAGATTGTCCGAG-3'

Mfn2: Mitofusin-2; Mcu: Mitochondrial Ca²⁺ uniporter; MICU: Mitochondrial Ca²⁺ uptake.

Citation: Liang RP, Jia JJ, Li JH, He N, Zhou YF, Jiang L, Bai T, Xie HY, Zhou L, Sun YL. Mitofusin-2 mediated mitochondrial Ca²⁺ uptake 1/2 induced liver injury in rat remote ischemic perconditioning liver transplantation and alpha mouse liver-12 hypoxia cell line models. World J Gastroenterol 2017; 23(38): 6995-7008
URL: https://www.wjgnet.com/1007-9327/full/v23/i38/6995.htm
DOI: https://dx.doi.org/10.3748/wjg.v23.i38.6995