Protective effects of oral glutathione on fasting-induced intestinal atrophy through oxidative stress

Figure 4 Effects of fasting and glutathione treatment on inducible nitric oxide synthase protein expression in the jejunum. A: Light micrographs of immunohistochemical staining for iNOS. In SA-treated groups, iNOS protein staining was localized almost exclusively in the mucosal epithelial monolayer with 48 and 72 h of fasting compared with the normally fed control. Decreased staining occurred with GSH treatment. Bar = 100 μm. B: Optical density of iNOS protein. The content of iNOS protein was quantitatively measured by averaging the optical density. Values represent the mean ± SE. ^bP < 0.01 vs normally fed controls. ^dP < 0.01, ^cP < 0.05 vs the respective SA group in each fasting period. 7-8 rats were tested in each group. GSH: Glutathione; SA: Saline; iNOS: Inducible nitric oxide synthase.