Attenuation of MET-mediated migration and invasion in hepatocellular carcinoma cells by SOCS1

Figure 1 Suppressor of cytokine signaling 1 attenuates hepatocyte growth factor-induced scattering and migration of hepatocellular carcinoma cells. A: Hepa and Hep3B cells expressing SOCS1 (Hepa-SOCS1, Hep3B-SOCS1) or control vector (Hepa-V, Hep3B-V) were grown to less than 50% confluent state, starved in serum-free medium for 16 h and exposed to murine or human HGF (HGF, 25 ng/mL), respectively. After 48 h, the culture plates were examined by phase contrast microscopy to visualize HGF-induced cell scattering; B: Hepa-SOCS1 and Hepa-V cells were seeded onto Matrigel-coated trans-well migration chambers, and migration towards m-HGF was evaluated after 48 h. Representative images of cells that had migrated across the membrane and the number of migrated cells in five random fields from three separate experiments (mean ± SE) are shown; C: Migration of Hep3B cells expressing SOCS1 (Hep3B-SOCS1) or control vector (Hepa-V) towards human HGF (h-HGF, 10 ng/mL). ^aP < 0.05, ^bP < 0.01,^eP < 0.001. SOCS1: Suppressor of cytokine signaling 1.