HOX transcript antisense intergenic RNA represses E-cadherin expression by binding to EZH2 in gastric cancer

Figure 4 HOTAIR represses E-cadherin expression by associating with EZH2. A: RIP experiments were performed in BGC-823 and SGC-7901 cells, and HOTAIR levels were analyzed in co-precipitated RNA using qPCR. The fold-enrichment of HOTAIR in EZH2 RIP is relative to its matching IgG control RIP (^aP < 0.05, ^bP < 0.01); B: ChIP of EZH2 and H3K27me3 in the E-cadherin promoter regions after siRNA treatment targeting si-NC or HOTAIR-siRNA-1 in BGC-823 and SGC-7901 cells. qPCR was performed to quantify ChIP assay results. Enrichment was quantified relative to input controls. Antibody directed against IgG was used as a negative control. Results are represented as the average ± SD based on three independent experiments. ^aP < 0.05, ^bP < 0.01; C: ChIP of EZH2 and H3K27me3 in the E-cadherin promoter regions after transfected with empty vector (denoted as ‘pcDNA’) or pcDNA-HOTAIR in BGC-823 and SGC-7901 cells. qPCR was performed to quantify ChIP assay results. Enrichment was quantified relative to input controls. Results are represented as the average ± SD based on three independent experiments (^aP < 0.05, ^bP < 0.01). ChIP: Chromatin immunoprecipitation; GC: Gastric cancer; HOTAIR: HOX transcript antisense intergenic RNA; qPCR: Quantitative polymerase chain reaction; RIP: RNA-binding protein immunoprecipitation; SD: Standard deviation; si: Small interfering.