HOX transcript antisense intergenic RNA represses E-cadherin expression by binding to EZH2 in gastric cancer

Figure 3 HOTAIR negatively correlates to E-cadherin expression in GC tissues and cell lines. A: E-cadherin immunostaining in para-carcinoma tissues (denoted as ‘P’) and GC tissues (denoted as ‘T’); B: Representative E-cadherin protein levels and scores in GC tissues and para-carcinoma tissues was analyzed by immunohistochemistry (n = 30); C: Negative correlation between E-cadherin mRNA levels and HOTAIR levels in 65 GC samples; D, E: Relative expression of HOTAIR and E-cadherin in BGC-823 and SGC-7901 cells treated with siRNA for 48 h; F, G: Western blot analysis of E-cadherin after HOTAIR-siRNA (Si1 and Si2) treatment for 48 h in BGC-823 and SGC-7901 cells. ^aP < 0.05, ^bP < 0.01. GC: Gastric cancer; HOTAIR: HOX transcript antisense intergenic RNA; si: Small interfering.