Changes in expression of inhibitory substances in the intramural neurons of the stomach following streptozotocin- induced diabetes in the pig

Table 2 The average percentage of neurons immunoreactive to bioactive substances used in this study

Stomach part	Control group			Experimental group
	Antrum	Corpus	Pylorus	Antrum	Corpus	Pylorus
	nNOS1			nNOS1
Myenteric ganglia	37.12 ± 2.81	22.28 ± 1.19	15.91 ± 0.58	41.93 ± 2.34	40.74b ± 2.22	35.38c ± 1.54
Submucosal ganglia	-	18.62 ± 1.66	-	-	19.79 ± 1.51	-
	VIP1			VIP1
Myenteric ganglia	18.38 ± 1.39	23.2 ± 0.23	23.64 ± 1.56	40.74c ± 1.77	30.93c ± 0.86	31.20a ± 1.10
Submucosal ganglia	-	43.61 ± 1.06	-	-	37.00a ± 1.77	-
	GAL1			GAL1
Myenteric ganglia	26.53 ± 1.52	17.73 ± 1.12	16.32 ± 0.43	36.67b ± 1.02	16.51 ± 0.92	17.99a ± 0.38
Submucosal ganglia	-	41.42 ± 0.88	-	-	40.49 ± 0.63	-

¹Relative frequency of particular neuronal subclasses is presented as percent (mean ± SE) of all neurons counted within the ganglia stained for Hu C/D proteins. nNOS, VIP and GAL immunoreactive perikarya in various parts of the porcine stomach under physiological conditions (Control group) and during experimentally induced hyperglicemia (Experimental group). Statistically significant data (^aP < 0.05,

^bP < 0.01, and

^cP < 0.001) in particular animal group are marked by superscripted alphabetical lettering. nNOS: Neuronal isoform of nitric oxide synthase; VIP: Vasoactive intestinal peptide; GAL: Galanin; Hu C/D: Pan-neuronal marker.