Copyright
©The Author(s) 2017.
World J Gastroenterol. Aug 28, 2017; 23(32): 5913-5924
Published online Aug 28, 2017. doi: 10.3748/wjg.v23.i32.5913
Published online Aug 28, 2017. doi: 10.3748/wjg.v23.i32.5913
Figure 2 Polyacrylamide gel resolution showing the 12G>C substitution in the 5’untranslated region after digestion.
Expected bands are 12, 44, 45 and 47 bp for all genotypes. Digestion of a sample with 2R or 3R12G genotype results in production of two bands of 66 and 28 bp, while in 3R12C genotypes those two fragments are left undigested in a single 94 bp fragment. bp: Base pair; LOH: Loss of heterozygosity; UTR: Untranslated region.
- Citation: Ntavatzikos A, Spathis A, Patapis P, Machairas N, Peros G, Konstantoudakis S, Leventakou D, Panayiotides IG, Karakitsos P, Koumarianou A. Integrating TYMS, KRAS and BRAF testing in patients with metastatic colorectal cancer. World J Gastroenterol 2017; 23(32): 5913-5924
- URL: https://www.wjgnet.com/1007-9327/full/v23/i32/5913.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i32.5913