(-)-Epigallocatechin-3-gallate enhances poly I:C-induced interferon-λ1 production and inhibits hepatitis C virus replication in hepatocytes

Figure 2 (-)-Epigallocatechin-3-gallate increases poly I:C-induced TLR3 and RIG-I mRNA expression in JFH-1-infected Huh7 cells. JFH-1-infected Huh7 cells (72 h post-infection) were treated with (-)-epigallocatechin-3-gallate (EGCG) at the indicated concentrations for 1 h prior to poly I:C (1 μg/mL) stimulation. Total RNA extracted from the cells after 24 h of stimulation was evaluated for TLR3 (A) and RIG-I (B) gene expression by real-time RT-PCR. The results shown are the mean ± SD of triplicate measurements representative of three experiments (Poly I:C vs LyoVec, ^aP < 0.05, ^bP < 0.01). TLR3: Toll-like receptor 3; RIG-I: Retinoic acid-inducible gene I.