Metabolomic profiling for identification of metabolites and relevant pathways for taurine in hepatic stellate cells

Table 3 Results of MetPA pathway analysis

Pathway name	Compounds1	Expected2	Hits	Raw P3	FDR P4	Impact
Glutathione metabolism	38	0.23681	2	0.022	0.896	0.272
Sphingolipid metabolism	25	0.1558	3	0.000	0.033	0.231
Thiamine metabolism	24	0.14956	1	0.140	1	0.125
Citrate cycle (TCA cycle)	20	0.12464	1	0.118	1	0.063
Valine, leucine and isoleucine biosynthesis	27	0.16826	1	0.156	1	0.013
Cysteine and methionine metabolism	56	0.34898	2	0.046	1	0.009
Glyoxylate and dicarboxylate metabolism	50	0.31159	1	0.271	1	0.003
Glycerophospholipid metabolism	39	0.24304	1	0.218	1	0.003

¹The total number of metabolites in each pathway;

²The actually matched number according to the uploaded data;

³The original P-value using enrichment analysis;

⁴The adjusted P-value using false discovery rate. The table shows the detailed results from the pathway analysis. Since we tested many pathways at the same time, the P-values from enrichment analysis are further adjusted for multiple tests. In particular, the Hits is the actually matched number from the user uploaded data; the Raw P is the original P-value calculated from the enrichment analysis; the FDR P is the P-value adjusted using false discovery rate; the Impact is the pathway impact value calculated from pathway topology analysis. Sixteen potential metabolic pathways were analyzed by MetPA network software and three significant metabolic pathways (Sphingolipid metabolism, glutathione metabolism and thiamine metabolism) were found to be the most important metabolic pathways for taurine in HSCs; HSCs: Hepatic stellate cells.