Autophagic cell death induced by reactive oxygen species is involved in hyperthermic sensitization to ionizing radiation in human hepatocellular carcinoma cells

Figure 1 Hyperthermia enhances the cytotoxicity of ionizing radiation to hepatocellular carcinoma cells. HepG2 cells were treated with hyperthermia (43 °C for 0.5 h) followed by ionizing radiation (4 Gy). After 72 h of incubation, the cells were assessed for cell viability using MTT assay (A), or plated in dishes and incubated for clonogenic survival assay (B). The results are presented as the mean ± SD of three different experiments. ^aP < 0.05 vs treatment of ionizing radiation alone.