Role of LAP+CD4+ T cells in the tumor microenvironment of colorectal cancer

Table 2 Primer sequences for polymerase chain reaction

Gene	Sequence (5’-3’)	Product (bp)	T (°C)
IL-2	F:5’ CAGCTACAACTGGAGCATTTAC	130	60
	R:5’ TCAGTTCTGTGGCCTTCTTG
IL-4	F:5’ GACCGTAACAGACATCTTTGC	180	60
	R:5’ TCGAGCCGTTTCAGGAAT
IL-10	F:5’ TTGCCAAGCCTTGTCTGA	160	60
	R:5’ ACAGGGAAGAAATCGATGAC
IL-17	F:5’ CCTCAGAGATCAACAGACCAA	80	60
	R:5’ GGTGCCTTGATCAGACAGAA
IFN-g	F:5’ GGCAAGGCTATGTGATTACA	180	60
	R:5’ TAAAGCACTGGCTCAGATTG
TGF-β1	F:5’ CACGTGGAGCTGTACCAGAA	219	60
	R:5’ GAACCCGTTGATGTCCACTT
18srRNA	F:5’ CCTGGATACCGCAGCTAGGA	112	60
	R:5’ GCGGCGCAATACGAATGCCCC