Copyright
©The Author(s) 2017.
World J Gastroenterol. Aug 7, 2017; 23(29): 5364-5370
Published online Aug 7, 2017. doi: 10.3748/wjg.v23.i29.5364
Published online Aug 7, 2017. doi: 10.3748/wjg.v23.i29.5364
Table 1 Condition of PCR and SSCP
| Primer set | PCR condition | SSCP temperature |
| (rs4268033) | ||
| 5'-TAATCCCAACTCGCAGCATCTGTGT-3' | 96 °C 15 s, 60 °C 30 s, 72 °C 30 s | 6 °C |
| 5'-GGTCTGACTTAGCTGGGGAAAGTGC-3' | 35 cycle | |
| (rs3735656) | ||
| 5'-ATCTCAGCGGACACAGGCAGGA-3' | 96 °C 15 s, 54 °C 40 s, 72 °C 30 s | 6 °C |
| 5'-CTGCACTGACCACAGTTGGTGAGAA-3' | 35 cycle | |
| (rs10226620) | ||
| 5'-GTCCCTCCTGTGACTGGGGTCTCT-3' | 96 °C 15 s, 60 °C 30 s, 72 °C 30 s | 6 °C |
| 5'-AGGCACCACCTTGCAGGTCTTATGT-3' | 35 cycle |
- Citation: Arisawa T, Nakamura M, Otsuka T, Jing W, Sakurai N, Takano H, Hayashi T, Ota M, Nomura T, Hayashi R, Shimasaki T, Tahara T, Shibata T. Genetic polymorphisms of MAFK, encoding a small Maf protein, are associated with susceptibility to ulcerative colitis in Japan. World J Gastroenterol 2017; 23(29): 5364-5370
- URL: https://www.wjgnet.com/1007-9327/full/v23/i29/5364.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i29.5364