Copyright
©The Author(s) 2017.
World J Gastroenterol. Aug 7, 2017; 23(29): 5324-5332
Published online Aug 7, 2017. doi: 10.3748/wjg.v23.i29.5324
Published online Aug 7, 2017. doi: 10.3748/wjg.v23.i29.5324
Table 2 List of primers used for reverse transcription and qRT-PCR
Name | Primer | Sequence (5’-3’) |
Reverse Universal Primer | GTGCAGGGTCCGAGGT | |
hsa-miR-125b-5p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGTCACAAGT |
Forward | TCCCTGAGACCCTAACTTG | |
hsa-miR-223-3p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGTGGGGTAT |
Forward | TGTCAGTTTGTCAAATACCC | |
hsa-miR-155-5p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCTAT |
Forward | TTAATGCTAATCGTGATAGG | |
hsa-miR-138-5p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGCGGCCTGA |
Forward | AGCTGGTGTTGTGAATCAG | |
hsa-miR-200a-3p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATCGTT |
Forward | TAACACTGTCTGGTAACGAT | |
hsa-miR-378d | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGTTTCTGTC |
Forward | ACTGGACTTGGAGTCAGAAA | |
Sno RNA U6 | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAAAATATG |
Forward | CAAATTCGTGAAGCGTTCCA |
- Citation: Valmiki S, Ahuja V, Paul J. MicroRNA exhibit altered expression in the inflamed colonic mucosa of ulcerative colitis patients. World J Gastroenterol 2017; 23(29): 5324-5332
- URL: https://www.wjgnet.com/1007-9327/full/v23/i29/5324.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i29.5324