Single amino acid mutation of SR-BI decreases infectivity of hepatitis C virus derived from cell culture in a cell culture model

Figure 4 Ability of the class B scavenger receptor I single amino acid mutant to bind to the hepatitis C virus E2 protein. CHO cells expressing the wild type (WT) and mutant SR-BI protein were incubated with equivalent amounts of hepatitis C virus E2 protein that had been expressed in HEK 293T cells for 1 h at RT, washed twice, and incubated with an anti-E2 mAb for 1 h at RT. After labeling with Alexa Fluor 488-conjugated goat anti-mouse IgG (Invitrogen), the mean fluorescence intensity was quantified by flow cytometry (^aP < 0.05). SR-BI: Class B scavenger receptor I.