Single amino acid mutation of SR-BI decreases infectivity of hepatitis C virus derived from cell culture in a cell culture model

Figure 2 Effect of the class B scavenger receptor I single amino acid mutant on hepatitis C virus infectivity. A: Immunofluorescence assay (IFA) of the effects of the SR-BI single amino acid mutant on hepatitis C virus (HCV) infectivity. Huh7-siSR-BI cells were seeded in 96-well plates, cultured overnight, and then transfected with pcDNA3.1 (NC), pcDNA-SR-BI (WT), or pcDNA- SR-BI/S112F. HCVcc (10³ FFU/mL) was added 24 h after transfection, and the IFA was performed 48 h later. B: Effect of the SR-BI single amino acid mutant on the HCV viral RNA. Huh7-siSR-BI cells were seeded in 24-well plates, cultured overnight, and then transfected with pcDNA3.1 (NC), pcDNA-SR-BI, or pcDNA-SR-BI/S112F. HCVcc (10⁴ FFU/mL) was added 24 h after transfection, and cells were harvested 72 h later to prepare RNA for the qRT-PCR analysis (^aP < 0.05). SR-BI: Class B scavenger receptor I; WT: Wild type.