Single amino acid mutation of SR-BI decreases infectivity of hepatitis C virus derived from cell culture in a cell culture model

Figure 1 Effect of the class B scavenger receptor I single amino acid mutant on hepatitis C virus entry. Huh7-siSR-BI cells were seeded on a 96-well plate, cultured overnight, and transfected with pcDNA3.1 (NC), pcDNA-SR-BI, or pcDNA-SR-BI/S112F. Three days after transfection, HCVpp were added to the cell culture. Cells were harvested 72 h post infection and cell lysates were analyzed using a fluorescence assay. The data are presented as the mean ± SEM from three independent experiments (^aP < 0.05). WT: Wild type.