miR-29a promotes hepatitis B virus replication and expression by targeting SMARCE1 in hepatoma carcinoma

Figure 2 Effect of miR-29a on hepatitis B virus replication and expression (n = 3). HepG2.2.15 cells were transfected with miR-control, miR-29a mimics or anti-miR-29a for 24 h. A: Relative HBV DNA copies were measured by qPCR analysis; B: HBV replication was detected by Southern blotting. The positions of relaxed circular (RC) and single-stranded (SS) DNAs are indicated 24 h post transfection. The ELISA assay was conducted to detect the levels of (C) HBsAg and (D) HBeAg 24 h post transfection. Data represent the mean ± SD. ^aP < 0.05 vs miR-control. HBV: Hepatitis B virus; HBsAg: Hepatitis B surface antigen; HBeAg: Hepatitis B e antigen.