Anti-inflammatory and anti-apoptotic effects of rosuvastatin by regulation of oxidative stress in a dextran sulfate sodium-induced colitis model

Figure 5 Rosuvastatin attenuated oxidative stress. IEC-6 cells were treated with 2 μmol/L rosuvastatin for 20 min or 50 ng/mL TNF-α and then stained with 25 μmol/L DCF-DA for 30 min. DCF fluorescence was detected by (A) confocal microscopy and (B) flow cytometry. Rosuvastatin significantly reduced ROS production. ^aP < 0.05 vs TNF-α -treated IEC-6 cells without rosuvastatin. TNF: Tumor necrosis factor; DCF-DA: 2'-7'-dichlorodihydrofluorescein diacetate; ROS: Reactive oxygen species; R: Rosuvastatin.