Indole phytoalexin derivatives induce mitochondrial-mediated apoptosis in human colorectal carcinoma cells

Figure 6 Effect of K-453 treatment on caspase activity and PARP cleavage. A: Activation of caspase-3; B: caspase-9 evaluated by flow cytometric analysis in HCT116 cells 24, 48 and 72 h after treatment; C: Analysis of caspase inhibition evaluated by MTS after 72 h treatment; D: Release of cytochrome c evaluated by flow cytometric analysis in HCT116 cells 24, 48 and 72 h after treatment; E: Cleavage of PARP evaluated by flow cytometric analysis 24, 48 and 72 h after treatment. Data represents the mean ± SD compared to the control of 3 independent experiments; F: Cleavage of PARP evaluated by Western blot analysis. Significance: ^aP < 0.05, ^bP < 0.01, ^dP < 0.001 vs untreated cells (control); ^eP < 0.05 vs ZVAD; ^fP < 0.01 vs K453.