Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation

Table 1 Details of primers used for real-time PCR analysis

Target	Sequence	Annealing, °C	Oligo concentration, nmol/L	Fragment size, bp	NCBI reference sequence	cDNA concentration, μL
GAPDH	F TGACTCTACCCACGGCAAGTTCAA	60	200	141	NM_017008.3	1.000
	R ACGACATACTCAGCACCAGCATCA
β-actin	F TTGCTGACAGGATGCAGAAGGAGA	60	100	159	NM_031144.2	1.000
	R ACTCCTGCTTGCTGATCCACATCT
HPRT	F AGGGAAGTGACAATCTACCTGACG	60	100	81	AA900579.1	0.125
	R GAAATGTCTGTTGCTGCGTCCCTT
HSP70	F ACTCCTTCGTTCGGTCTGCAATCA	60	200	92	NM_031971.2	0.125
	R CTGGGAATGCAAAGCACACGTGAA
Heparanase	F TGTCAAGAGTGAAAGGCCCAGACA	60	200	141	NM_022605.1	0.125
	R GCAGCTTCAAGTGCTTGGTGACAT
NF-κB	F AAACCAAAGCCCTGAAAGGCCATC	60	200	120	XM_342346.4	0.125
	R TCGGAAGGCCTCGAATGACATCAA
MAPK1	F AACAGGTTGTTCCCAAACGCTGAC	60	200	187	NM_053842.1	0.500
	R AGTCGTCCAGCTCCATGTCAAACT
MAPK3	F TACCTGGACCAGCTCAACCACATT	60	200	173	NM_017347.2	0.500
	R AGCAGGTCAAGAGCTTTGGAGTCA
MAPK6	F AACTGAGCCAGTGGAAGAAGGGAA	60	200	164	NM_031622.2	1.000
	R TTAACGTGGCCTGGATGGACTTGA
MAPK9	F TCATGGGAGAGCTGGTGAAAGGTT	60	200	106	NM_017322.2	1.000
	R ATGAACTCTGCGGATGGTGTTCCT				NM_001270544.1
					NM_001270545.1
MUC1	F CCGCTACTACCAAGAACTGAAG	60	200	102	NM_012602.1	0.500
	R GAGCCTGACCTGAACTTGATAG
MUC2	F GGCTCTGCTCTCTGTGTTATAG	60	200	123	U68172.1	1.000
	R CAGTTTGGGAAGAAGGTAGGG
MUC3	F GGGAAATAGACCCTGCAGTTAG	60	200	107	U76551.1	0.125
	R GATCATCGCTTGCCGTCATA
MUC4	F ATGTGGAGGTGGGAGAAATG	60	200	122	AF240632.1	0.500
	R CCCTGGAACTGGAATTAGAGAC