Indole phytoalexin derivatives induce mitochondrial-mediated apoptosis in human colorectal carcinoma cells

Table 5 Annexin V/PI flow cytometry analysis of apoptosis occurrence in HCT116 cells after K-453 treatment (in %)

Treatment	Time (h)	An-/PI-	An+/PI-	An+/PI+
Control	24	89.8 ± 1.41	4.46 ± 0.35	1.82 ± 1.03
	48	92.45 ± 0.07	2.38 ± 0.45	1.71 ± 0.25
	72	94.55 ± 0.92	2.13 ± 0.59	1.51 ± 0.31
K-453	24	66.35 ± 7.0b	23.4 ± 3.25b	6.38 ± 6.84
	48	37.85 ± 9.12c	34.75 ± 4.88c	11.43 ± 7.88a
	72	36.05 ± 14.07c	30.25 ± 11.1c	10.2 ± 3.39a

The results are presented from 3 independent experiments. Living cells are presented as An^-/PI^- events, cells in a stage of early apoptosis are presented as An⁺/PI^-, late apoptotic and necrotic cells as An⁺/PI⁺. Significantly different,

^aP < 0.05,

^bP < 0.01,

^cP < 0.001 vs untreated cells (control).