Indole phytoalexin derivatives induce mitochondrial-mediated apoptosis in human colorectal carcinoma cells

Table 4 Flow cytometric analysis of cell cycle distribution in HCT116 cells treated with compound K-453 (in %)

Treatment	Time (h)	sub-G1	G0/G1	S	G2/M
Control	24	1.30 ± 0.07	48.38 ± 0.81	23.28 ± 0.53	27.05 ± 0.35
	48	1.75 ± 0.22	52.1 ± 2.12	22.88 ± 0.08	23.30 ± 2.26
	72	1.64 ± 0.09	48.57 ± 0.52	24.85 ± 0.64	24.95 ± 0.21
K-453	24	19.7 ± 2.26b	62.97 ± 0.37b	7.05 ± 1.35b	10.29 ± 1.29b
	48	48.9 ± 7.21c	35.58 ± 3.22a	9.31 ± 2.54b	6.22 ± 1.45b
	72	55.11 ± 14.13c	34.11 ± 9.89a	5.71 ± 1.73b	5.07 ± 2.52b

The results are presented from three independent experiments as the mean ± SD; significantly different,

^aP < 0.05,

^bP < 0.01,

^cP < 0.001 vs untreated cells (control); sub-G1 fraction of cells identified as apoptotic population.