Copyright
©The Author(s) 2017.
World J Gastroenterol. Jun 28, 2017; 23(24): 4341-4353
Published online Jun 28, 2017. doi: 10.3748/wjg.v23.i24.4341
Published online Jun 28, 2017. doi: 10.3748/wjg.v23.i24.4341
Table 1 Flow cytometry staining
Analysis | Staining solution | Manufacturer |
Caspase activation | Caspase-3-PE | BD Biosciences Pharmingen, San Diego, CA, United States |
Cleaved Caspase-9-FITC | BIOSS Antibodies, Woburn, MA, United States | |
Cytochrome c release | Cytochrome c Antibody (6H2) FITC Conjugate | Invitrogen, Carlsbad, CA, United States |
Cleavage of PARP | Cleaved-PARP (Asp214) XP® Rabbit mAb (PE Conjugate) | Cell Signaling Technology, Danvers, MA, United States |
Mitochondrial membrane potential | TMRE (tetramethylrhodamine ethyl ester perchlorate) Final concentration 0.1 µmol/L | Sigma-Aldrich, St. Louis, Missouri, United States |
Protein analysis | Phospho-Bcl-2 (Ser70) Rabbit mAb Alexa Fluor® 488 Conjugate | Cell Signaling Technology®, Danvers, MA, United States |
Phospho-Bad (Ser112) Rabbit mAb (PE Conjugate) |
- Citation: Tischlerova V, Kello M, Budovska M, Mojzis J. Indole phytoalexin derivatives induce mitochondrial-mediated apoptosis in human colorectal carcinoma cells. World J Gastroenterol 2017; 23(24): 4341-4353
- URL: https://www.wjgnet.com/1007-9327/full/v23/i24/4341.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i24.4341