Indole phytoalexin derivatives induce mitochondrial-mediated apoptosis in human colorectal carcinoma cells

doi:10.3748/wjg.v23.i24.4341

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Jun 28, 2017 (publication date) through Jul 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 28, 2017; 23(24): 4341-4353
Published online Jun 28, 2017. doi: 10.3748/wjg.v23.i24.4341

Table 1 Flow cytometry staining

Analysis	Staining solution	Manufacturer
Caspase activation	Caspase-3-PE	BD Biosciences Pharmingen, San Diego, CA, United States
Caspase activation	Cleaved Caspase-9-FITC	BIOSS Antibodies, Woburn, MA, United States
Cytochrome c release	Cytochrome c Antibody (6H2) FITC Conjugate	Invitrogen, Carlsbad, CA, United States
Cleavage of PARP	Cleaved-PARP (Asp214) XP^® Rabbit mAb (PE Conjugate)	Cell Signaling Technology, Danvers, MA, United States
Mitochondrial membrane potential	TMRE (tetramethylrhodamine ethyl ester perchlorate) Final concentration 0.1 µmol/L	Sigma-Aldrich, St. Louis, Missouri, United States
Protein analysis	Phospho-Bcl-2 (Ser70) Rabbit mAb Alexa Fluor^® 488 Conjugate	Cell Signaling Technology^®, Danvers, MA, United States
Protein analysis	Phospho-Bad (Ser112) Rabbit mAb (PE Conjugate)	Cell Signaling Technology^®, Danvers, MA, United States

Citation: Tischlerova V, Kello M, Budovska M, Mojzis J. Indole phytoalexin derivatives induce mitochondrial-mediated apoptosis in human colorectal carcinoma cells. World J Gastroenterol 2017; 23(24): 4341-4353
URL: https://www.wjgnet.com/1007-9327/full/v23/i24/4341.htm
DOI: https://dx.doi.org/10.3748/wjg.v23.i24.4341