Green tea polyphenols ameliorate non-alcoholic fatty liver disease through upregulating AMPK activation in high fat fed Zucker fatty rats

Figure 4 Effects of green tea polyphenols on de novo lipogenesis pathway in the liver of Zucker fatty rats. Proteins extracted from the livers of lean, Zucker fatty (ZF)-Con. and ZF-GTP rats were separated by SDS-PAGE and immunoblotted with anti-p-AMPK-α, anti-AMPK-α, and anti-p-ACC: Anti-ACC. β-Actin was used to confirm equal protein loading. Upper panels (A and C): Immunoblot bands of detected proteins; lower panels (B and D): Quantitative analysis of corresponding proteins. Data are expressed as mean ± SME (n = 8). ^aP < 0.05 vs lean, ^cP < 0.05 vs ZF-Con. GTP: Green tea polyphenols; ACC: Acetyl-CoA carboxylase.