Green tea polyphenols ameliorate non-alcoholic fatty liver disease through upregulating AMPK activation in high fat fed Zucker fatty rats

Figure 2 Reduction of hepatic steatosis and triglyceride content with green tea polyphenols treatment. A: Images were stained with H&E to visualise macrovesicular steatosis in liver sections at × 400 magnification. Compared to the livers in lean control rats, a larger unstained area that consisted of fat vacuoles was seen in ZF control rats; B: Representative ORO staining of liver samples from lean, ZF-Con and ZF-GTP rats. A larger red area that consisted of lipid droplets (B, C: ORO staining; × 400 magnification) and higher triglyceride contents (D) in the livers of ZF-control rats. GTP treatment significantly reversed these changes in the liver. Data are expressed as mean ± SME from 6-8 rats each group. ^bP < 0.01, ^eP < 0.001 vs Lean group; ^dP < 0.01 vs ZF-Con group. GTP: Green tea polyphenols; ZF: Zucker fatty.