Effect of NDC80 in human hepatocellular carcinoma

Figure 5 Effects of the silencing of NDC80 on SMMC-7721 cell cycle distribution and apoptosis. A: After lentiviral transfection of SMMC-7721 cells, cell cycle assessment showed that knockdown of NDC80 in SMMC-7721 cells induced accumulation in S-phase. The percentages of cells in different phases are shown as the mean ± SD of three independent experiments; B: Apoptotic rates were analyzed by Annexin V-FITC/PI assay. Apoptosis was significantly increased in NDC80-siRNA-silenced cells as compared to control cells. Statistical significance was assessed by two-tailed Student’s t test. Error bar indicates SD (^eP < 0.05; ^cP < 0.01; ^aP < 0.0001, vs control).