Effect of NDC80 in human hepatocellular carcinoma

Figure 2 Interference efficiency 72 h after transfection. A: After lentiviral transfection, relative NDC80 mRNA expression was significantly inhibited in the SMMC-7721 NDC80-siRNA silenced cells as compared to SMMC-7721 negative control cells by RT-PCR; B: Western blotting of NDC80-depletion efficiency in SMMC-7721 cells; C: Gray value analysis of western blotting, and data were normalized against GAPDH. GAPDH was used as an internal control. Statistical significance was assessed by two-tailed Student’s t test. Error bar indicates SD (^cP < 0.01 vs control; ^aP < 0.001 vs control).