Effect of NDC80 in human hepatocellular carcinoma

Figure 1 Reverse transcription polymerase chain reaction results for NDC80 mRNA expression. A: Expression levels of NDC80 mRNA in HCC (n = 47) and paired adjacent tissue samples (n = 47); B: NDC80 protein expression in HCC and paired adjacent tissue samples was determined by western blotting; C: Gray value analysis of western blot experiments, and data was normalized against GAPDH; D: NDC80 mRNA expression varied among SMMC-7721, HepG2, Hep3B and Hun-7 cell lines. GAPDH was used as an internal control. Statistical significance was assessed by paired t tests. Error bar indicates SD (^aP < 0.001 vs control).