Copyright
©The Author(s) 2017.
World J Gastroenterol. Jan 14, 2017; 23(2): 242-255
Published online Jan 14, 2017. doi: 10.3748/wjg.v23.i2.242
Published online Jan 14, 2017. doi: 10.3748/wjg.v23.i2.242
Figure 1 Intracolonic administration of adeno-associated virus carrying thymosin β4 mediated secretory expression of thymosin β4 in mouse colons.
Two weeks after intracolonic (ic) adeno-associated virus (AAV)-LacZ, AAV-thymosin β4 (Tβ4) or PBS, the mice were euthanized to harvest their colons. A: Real-time PCR showed the presence of viral DNA in the colonic tissues of the AAV-treated mice; B: X-gal staining verified AAV-mediated expression of LacZ in the colonic tissues. C: Immunohistochemistry revealed that ic AAV-Tβ4 resulted in markedly increased expression of Tβ4 in the mouse colon; D: In the mice with experimental colitis, Western blot showed that dextran sulfate sodium (DSS) treatment remarkably upregulated the colonic Tβ4 expression while TNBS enemas only slightly increased colonic Tβ4. Intracolonic AAV-Tβ4 led to substantially increased expression of Tβ4 in the colonic tissues in both DSS- and TNBS-treated mice. Error bars indicate SD. Scale bars = 50 μm.
- Citation: Zheng XY, Lv YF, Li S, Li Q, Zhang QN, Zhang XT, Hao ZM. Recombinant adeno-associated virus carrying thymosin β4 suppresses experimental colitis in mice. World J Gastroenterol 2017; 23(2): 242-255
- URL: https://www.wjgnet.com/1007-9327/full/v23/i2/242.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i2.242