Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

Figure 3 Preparation of the reporter gene GFPCreERT2. A: Diagram of the shuttle vector carrying GFPCreERT2; B: Electrophoretogram of the shuttle vector cut by NotI and FseI; C: Electrophoretogram after the GFPCreERT2 fragment was cut from the gel. M: Marker; Gpm6a: Shuttle vector inserted by the 5arm and 3arm from the Gpm6a BAC; Reelin: Shuttle vector inserted by the 5arm and 3arm from the Reelin BAC.