Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

Figure 1 Confirmation of bacterial artificial chromosome DNA with restriction enzyme digestion. A: Electrophoretogram of the original BAC with BamHI digestion; B: The expected bands of the original BAC with BamHI digestion. The color of the number is same as the arrow. M: Marker; Uncut: Original BAC without digestion; 5, 10, 20: 5 μg, 10 μg, 20 μg of original BAC with BamHI digestion. BAC: Bacterial artificial chromosome.