Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease

Figure 1 LiverChip^® three-dimensional perfused cell culture system. A: The LiverChip^® plate contains 12 independent bioreactors for the 3D culture of hepatocytes; B: Hepatocytes form 3D micro-tissue structures in an array of channels in a collagen I-coated scaffold that is contained in each LiverChip^® well; C: Schematic representation of how media flows through the channels of each LiverChip scaffold due to the action of a pneumatically operated pumps in the base of the plate. The speed and direction of flow can be adjusted using an electronic controller. 3D: Three-dimensional.