Diabetes recurrence after metabolic surgeries correlates with re-impaired insulin sensitivity rather than beta-cell function

Figure 5 Pancreatic beta cell analysis. A: TUNEL-positive beta cell shown by immunofluorescence; B: and the percentage of TUNEL-positive beta cells was calculated. The two surgery group had fewer TUNEL-positive beta cells than the Sham group, and HFD group made the percentage higher than in the LFD group; C: The fluorescence intensity did not differ significantly between the four surgery groups (P > 0.05), but was higher than in the Sham group. ^aP < 0.05 vs Sham group at 12 wk postoperatively; ^cP < 0.05 vs SG-LFD group at 12 wk postoperatively; ^eP < 0.05 vs DJB-LFD group at 12 wk postoperatively. TUNEL: Transferase-mediated dUTP-biotin 3’ nick end-labeling; SG: Sleeve gastrectomy; SG-LFD: SG rats with common low-fat diet; SG-HFD: SG with high-fat diet; DJB: Duodenal-jejunal bypass; DJB-LFD: DJB rats with LFD; DJB-HFD: DJB rats with HFD.