Effects of heme oxygenase-1-modified bone marrow mesenchymal stem cells on microcirculation and energy metabolism following liver transplantation

Figure 7 Endothelial nitric-oxide synthase expression in liver after liver transplantation. A: Immunohistochemistry (IHC) of endothelial nitric-oxide synthase (eNOS); B: IHC integrated optical density (IOD) of eNOS; C: Western blotting and eNOS protein levels. eNOS protein levels: postoperative day (POD) 1: HO-1/BMMSCs group vs BMMSCs group vs NS group: 0.79 ± 0.14 vs 0.42 ± 0.02 vs 0.25 ± 0.02 (P < 0.05). POD5: HO-1/BMMSCs vs NS group: 0.84 ± 0.04 vs 0.50 ± 0.01 (P < 0.01); HO-1/BMMSCs group vs BMMSCs group: 0.84 ± 0.04 vs 0.50 ± 0.01 (P < 0.05). POD7: HO-1/BMMSCs group vs BMMSCs group vs NS group: 1.00 ± 0.12 vs 0.84 ± 0.06 vs 0.50 ± 0.01 (P < 0.05). POD14: HO-1/BMMSCs group vs BMMSCs group vs NS group: 0.90 ± 0.09 vs 0.77 ± 0.07 vs 0.48 ± 0.02 (P < 0.01). ^aP < 0.05 vs NS group, ^cP < 0.05 vs HO-1/BMMSCs group.