Effects of heme oxygenase-1-modified bone marrow mesenchymal stem cells on microcirculation and energy metabolism following liver transplantation

Figure 6 Endothelin-1/nitrous oxide related factors expression in serum after liver transplantation. A: Expression of endothelin-1 (ET-1); B: Expression of endothelial nitric-oxide synthase (eNOS); C: Expression of inducible nitric-oxide synthase (iNOS); D: Expression of NO. The serum ET-1 after RLT decreased initially and then increased with the extension of post-operative time. Postoperative day (POD) 1: HO-1/BMMSCs group vs NS group: 24.53 ± 1.22 vs 29.29 ± 2.97 (P < 0.01). POD5: HO-1/BMMSCs vs BMMSCs vs NS group: 28.84 ± 0.17 vs 32.18 ± 0.30 vs 36.27 ± 0.92 (P < 0.01). POD7: HO-1/BMMSCs group vs BMMSCs group vs NS group: 29.92 ± 0.28 vs 31.87 ± 0.50 vs 36.25 ± 0.54 (P < 0.01). POD14: HO-1/BMMSCs group vs NS group: 33.82 ± 1.44 vs 40.03 ± 0.17 (P < 0.01); HO-1/BMMSCs group vs BMMSCs group: 33.82 ± 1.44 vs 38.35 ± 2.09 (P < 0.01). The serum levels of eNOS increased with the extension of post-operative time. POD1: HO-1/BMMSCs group vs NS group: 5.86 ± 0.30 vs 4.30 ± 0.07 (P < 0.01); HO-1/BMMSCs group vs BMMSCs group: 5.86 ± 0.30 vs 4.74 ± 0.54 (P < 0.01). POD5: HO-1/BMMSCs vs BMMSCs vs NS group: 7.39 ± 0.29 vs 6.36 ± 0.07 vs 5.27 ± 0.16 (P < 0.01). POD7: HO-1/BMMSCs group vs BMMSCs group vs NS group: 7.43 ± 0.19 vs 6.17 ± 0.12 vs 5.37 ± 0.12 (P < 0.01). POD14: HO-1/BMMSCs group vs NS group: 6.72 ± 0.30 vs 5.40 ± 0.15 (P < 0.01); HO-1/BMMSCs group vs BMMSCs group: 6.72 ± 0.30 vs 5.26 ± 0.20 (P < 0.01). The serum levels of iNOS increased with the extension of post-operative time, and was most significant in NS group. POD1: HO-1/BMMSCs group vs NS group: 8.57 ± 0.07 vs 10.24 ± 0.42 (P < 0.01); BMMSCs group vs NS group: 8.56 ± 0.20 vs 10.24 ± 0.42 (P < 0.01). POD5: HO-1/BMMSCs vs BMMSCs vs NS group: 9.06 ± 0.05 vs 9.97 ± 0.08 vs 15.66 ± 0.13 (P < 0.01). POD7: HO-1/BMMSCs group vs NS group: 9.87 ± 0.42 vs 15.96 ± 0.74 (P < 0.01); BMMSCs group vs NS group: 10.85 ± 0.90 vs 15.96 ± 0.74 (P < 0.01). POD14: HO-1/BMMSCs group vs NS group: 10.04 ± 0.61 vs 16.85 ± 0.31 (P < 0.05); BMMSCs group vs NS group: 10.73 ± 0.64 vs 16.85 ± 0.31 (P < 0.01). The serum levels of NO tended to increase initially and then decrease with the extension of post-operative time. POD1: HO-1/BMMSCs group vs NS group: 10.64 ± 0.36 vs 7.92 ± 0.59 (P < 0.01); HO-1/BMMSCs group vs BMMSCs group: 10.64 ± 0.36 vs 8.56 ± 0.44 (P < 0.01). POD5: HO-1/BMMSCs vs BMMSCs vs NS group: 9.17 ± 0.08 vs 9.65 ± 0.09 vs 10.50 ± 0.07 (P < 0.01). POD7: HO-1/BMMSCs group vs NS group: 9.19 ± 0.12 vs 10.00 ± 0.10 (P < 0.01); BMMSCs group vs NS group: 9.41 ± 0.39 vs 10.00 ± 0.10 (P < 0.01). ^aP < 0.05 vs NS group, ^cP < 0.05 vs HO-1/BMMSCs group.