Role of AXL in invasion and drug resistance of colon and breast cancer cells and its association with p53 alterations

Figure 2 Invasion assays of different RKO clones. A: The base line invasion of RKO.p53^-/-, which was not silenced or exposed to treatment, was high, as indicated; B: The same cell line after AXL siRNA silencing shows some decreased invasion; C: RKO.p53^-/- treated with siRNA mock control has increased invasiveness and showed some aggressive cells with increased nuclear size, clumped chromatin, increased cytoplasmic extensions (arrow), after exposure to 5FU treatment; D: Quantitative evaluation of different cells after dye elution and spectrophotometric reading at 560 nm.