Oxidative stress-induced mitochondrial dysfunction in a normal colon epithelial cell line

Figure 5 Increased autophagy observed in CRL. 1790 cells with microbial stimulation. A and B: CRL.1790 cells stimulated with heat killed cecal contents (HKC) or heat killed E. coli for 6 h and 12 h were assessed for presence of autophagic vesicles using ENZO cytoID (n = 3). Chloroquine and rapamycin treated cells were used as positive control; C and D: Representative images showing staining of autophagic vesicles (magnification × 40). ^bP < 0.01 vs Control. Data are expressed as mean ± SE. CTCF: Corrected total cell fluorescence.