Oxidative stress-induced mitochondrial dysfunction in a normal colon epithelial cell line

Figure 3 Mitochondrial dysfunction in CRL. 1790 cells is evident during microbial stimulation. CRL.1790 cells stimulated with heat killed cecal contents (HKC) or heat killed E. coli for 6 h and 12 h were assessed for mitochondrial function using mitotracker staining. A and B: Denotes average circularity of mitochondria assessed 6 h and 12 h post stimulation respectively; C and D: Denotes intensity of mitotacker staining in cells 6 h and 12 h post stimulation respectively; E and F: Expression of DRP1 and MFN2 in CRL.1790 cells treated with HKC or E.coli for 6 h and 12 h post stimulation respectively; G: Representative images showing mitotracker staining (n = 3) (magnification × 60); H and I: Representative images showing expression of Drp1 and MFN2 (magnification × 60). ^bP < 0.01 vs Control. Data are expressed as mean ± SE. CTCF: Corrected total cell fluorescence; CCCP: Carbonyl cyanide 3-cholorphenylhydrazone.