Oxidative stress-induced mitochondrial dysfunction in a normal colon epithelial cell line

Figure 2 CRL. 1790 cells generates robust reactive oxygen species upon microbial stimulation. CRL.1790 cells were stimulated with heat killed cecal contents (HKC) or heat killed E. coli for 6 h and 12 h. A: Fluorescence intensity of the CRL.1790 cells preloaded with carboxy-H2DCFDA dye; B: CellROX staining intensity (CTCF- corrected total cell fluorescence) was measured to determine ROS generation in CRL.1790 cells (n = 3). ^bP < 0.01 vs Control. Data are expressed as mean ± SE. ROS: Reactive oxygen species; CTCF: Corrected total cell fluorescence.