Role of AXL in invasion and drug resistance of colon and breast cancer cells and its association with p53 alterations

Table 2 Cell lines origin and p53 status

Cell lines	Cell line description1	p53 status1
Breast cancer cell lines
MCF7	Adenocarcinoma of the mammary gland, derived from 69-year-old female, cells were obtained from metastatic site; pleural effusion	Wild-type[34]
1001	1001 is derived from its parental MCF7 (MCF7/R-A1; which are cells exposed to increasing dose of recombinant TNF, transfected by p55 TNF receptor cDNA, Mutation in R280K)[35]	TNF resistant associated with loss of p53 function[35]
CAL51	Adenocarcinoma isolated from a malignant pleural effusion of a 44-year-old female with metastatic breast cancer, normal karyotype with genetic stability[36]	Wild-type[37]
ZR-75-1	Ductal carcinoma of the mammary gland, derived from 63-year-old female, cells were obtained from metastatic site: ascites	Wild-type
MDA-MB-361	Adenocarcinoma of the mammary gland, derived from 40-year-old female, cells were obtained from metastatic site; Brain	Wild-type
T47D	ductal carcinoma of the mammary gland, derived from 54-year-old female, cells were obtained from metastatic site; pleural effusion	Heterozygous mutant.
MDA-MB-231	Adenocarcinoma of the mammary gland, derived from 51-year-old female, cells were obtained from metastatic site; pleural effusion.	Homozygous mutant
BT549	Ductal carcinoma of the mammary gland, derived from 72-year-old female, cells were obtained from mammary gland.	Homozygous mutant
Colon Cancer Cell lines
RKO	poorly differentiated colon carcinoma cell line developed by Michael Brattain	Wild-type.
RKO-p53	RKO parental cell line double negative mutant for p53	Homozygous knock out2
HCT 116	Colorectal carcinoma of Adult Male derived from primary tumor site	Wild-type
HCT 116.p53	HCT116 parental cell line with hemizygous p53 mutation and knock out of the homologous p53	R284w/-2
SW480	Dukes' type B, colorectal adenocarcinoma, derived from 50-year-old male, cells obtained from primary tumor site	Homozygous mutant
Positive control for EMT
HeLa	Cervical adenocarcinoma of 31-year-old female derived from primary tumor site	Wild-Type but HPV inactivated[34]

¹The cell line origin and the p53 status were from ATCC data sheets/website, the Cancer Cell Line Encyclopedia or the indicated references;

²As per the source/supplier: Horizon Discovery, Cambridge, United Kingdom.