Thiopurine use associated with reduced B and natural killer cells in inflammatory bowel disease

Figure 5 Intestinal B, but not T cells, are reduced in thiopurine recipients. Collagenase-digested colon biopsies from healthy controls (open grey circles) vs inflammatory bowel disease patients on (black triangles) vs not on thiopurines (grey squares) underwent flow cytometry. The percent of lymphocytes expressing the T cell marker CD3 (A) and the CD4:CD8 ratio thereof (B) is shown for each cohort. The percent of lymphocytes expressing the B cell marker CD19 (C) and the CD27-, CD38+, IgD+ or CD27-, CD38-, IgD+ fractions thereof resembling transitional (D) and naïve (E) B cells, respectively, are shown. The fraction of CD27+, IgD-, CD20+, CD38- memory B cells (F) and CD27+, CD38+, CD20-, IgD- plasma cells (G) expressing surface IgM is shown. P values reflect two-tailed Mann-Whitney non-parametric comparisons.