Low-grade slightly elevated and polypoid colorectal adenomas display differential β-catenin-TCF/LEF activity, c-Myc, and cyclin D1 expression

Figure 3 β-catenin pathway analysis. A: Western blotting revealed that β-catenin protein expression was significantly upregulated in slightly elevated lesions relative to polypoid lesions. B: A dual-luciferase reporter assay using an STF construct (containing a firefly luciferase reporter driven by several TCF/LEF consensus binding sites) revealed significant upregulation of β-catenin-TCF/LEF-driven transcriptional activity in slightly elevated lesions relative to polypoid lesions. ^aP < 0.05, slightly elevated vs polypoid.