High throughput RNA sequencing utility for diagnosis and prognosis in colon diseases

Figure 1 RNA sequencing steps. A: RNA isolation techniques include the usage of beads with poly(T) primer tails to isolate mRNA or gel electrophoresis to isolate smaller RNA molecules; B: RNA libraries are prepared by attaching adaptors to isolated RNA strands, creating cDNA strands corresponding to the isolated RNA strands, amplifying the cDNA, and then fragmenting the cDNA strands; C: High throughput cDNA sequencing involves sequencing cDNA fragments in parallel by nucleotide addition; D: cDNA sequences are then analyzed by matching fragments onto known genomes or de novo mapping to produce a transcriptome.