Overexpression of fibrinogen-like protein 2 protects against T cell-induced colitis

Figure 2 Isolation of CD4⁺CD25⁺CD45RB^low Treg and CD4⁺CD25^-CD45RB^high Teff. A: Representative flow plots of CD4 vs CD25 populations. SMNC were isolated and enriched for CD4⁺ T cells by negative T cell selection using magnetic cell sorting. CD4⁺ T cells fractions were stained with CD4⁺-PE-Cy7, CD25⁺-PE and CD45RB-APC and sorted into CD4⁺CD25⁺CD45RB^low and CD4⁺CD25^-CD45RB^high T cell fractions. Cells were gated on live cells, singlets and CD4⁺ populations. Plots show initial SMNC population and the CD4⁺CD25⁺ and CD4⁺CD25^- T cell populations following FACS sorting; B: Histogram of CD45RB cell distribution; C: Flow plots of CD25 vs CD45RB populations. Final populations of CD4⁺CD25⁺CD45RB^low and CD4⁺CD25^-CD45RB^high T cell fractions were > 98% pure. SMNC: Splenic mononuclear cells; Teff: Effector T cell; Treg: Regulatory T cell.