Overexpression of fibrinogen-like protein 2 protects against T cell-induced colitis

Figure 1 Immunologic characterization of fgl2^Tg mice. A: Fibrinogen-like protein 2 (FGL2) plasma levels. Bar represents mean levels (n = 7-8 mice/group); B: One-way MLR and FGL2 levels in MLR culture supernatants. FGL2 overexpression inhibits T cell proliferation in response to stimulation with BALB/c alloantigens. Proliferation was measured by flow cytometry. FGL2 levels in MLR culture supernatant were measured by an FGL2 ELISA. Data represent the mean ± SD and are representative of three independent experiments; C: Foxp3⁺ cell percentages in the thymus, spleen and lymph nodes. Foxp3⁺ cells are represented as a percentage of total SMNC. Data are expressed as the mean ± SD (n = 3 mice/group); D: Treg suppression assay. The suppressive activity of Treg is expressed as a percent inhibition of T cell proliferation compared to responder T cells alone. Graphs show the mean ± SD. Data are representative of 3 independent experiments. (^aP < 0.05, ^bP < 0.01, ^eP < 0.001). MLR: Mixed lymphocyte reaction; SMNC: Splenic mononuclear cells; Treg: Regulatory T cell.